Placental mesenchymal stem cell derived-extracellular vesicles (PMSC-EVs) triggercellular regeneration with less toxicity andimmunogenicity compared to cell-basedtherapy. Conventional monolayer cell culture has low yield of PMSC-EVs which limits currentapplications.
The CELLine bioreactor, allows for a high-density 3D cell culture within a semipermeablemembrane. It has been utilized as a large-scaletissue culture method. The objective is to explore the application of the CELLine bioreactor as a novel approach to improve the production and yield of PMSC-EVs for regenerative medicine applications.
Spinal cord injuries (SCI) are devastating with no effective treatments and excessive medical costs. Placental mesenchymal stem cells (PMSCs) are a promising avenue of treatment due to their neuroprotective and angiogenic properties (Kumar, 2019). In addition, extracellular vesicles (EVs) released from these stem cells are an attractive cell-free therapeutic approach. Extracellular vesicles are approximately 150 nm vesicles released by placental mesenchymal stem cells that contain the proteins, miRNAs and other cargo of the cells themselves (Ramasubramanian 2019).
CDH is a congenital defect in the diaphragm causing herniation of abdominal contents and impaired lung growth. PTFE is a synthetic polymer that is the current standard of care for patch repair of CDH. Limitations: stiff, not biodegradable, no growth with the child leading to reherniation and repeated intervention. Polyurethane (PU) is a novel material that is elastic, biodegradable, and biocompatible, allowing for optimized lung mechanics and tissue integration.
The role of supraspinal circuits in the control posture after spinal cord injury (SCI) is poorly understood. Understanding how the brain encodes such information before and after SCI can inform the design a motor brain-machine interface for restoration of motor function.
• The role of supraspinal circuits in the control posture after spinal cord injury (SCI) is poorly understood. Understanding how the brain encodes such information before and after SCI can inform the design a motor brain-machine interface for restoration of motor function.
