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Three-dimensional culture system improves the yield of placentalmesenchymal stem cell-derived extracellular vesicles
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Abstract
Placental mesenchymal stem cell derived-extracellular vesicles (PMSC-EVs) triggercellular regeneration with less toxicity andimmunogenicity compared to cell-basedtherapy. Conventional monolayer cell culture has low yield of PMSC-EVs which limits currentapplications.
The CELLine bioreactor, allows for a high-density 3D cell culture within a semipermeablemembrane. It has been utilized as a large-scaletissue culture method. The objective is to explore the application of the CELLine bioreactor as a novel approach to improve the production and yield of PMSC-EVs for regenerative medicine applications.
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