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Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
- Zhang, Jason Z;
- Yeh, Hsien-Wei;
- Walls, Alexandra C;
- Wicky, Basile IM;
- Sprouse, Kaitlin R;
- VanBlargan, Laura A;
- Treger, Rebecca;
- Quijano-Rubio, Alfredo;
- Pham, Minh N;
- Kraft, John C;
- Haydon, Ian C;
- Yang, Wei;
- DeWitt, Michelle;
- Bowen, John E;
- Chow, Cameron M;
- Carter, Lauren;
- Ravichandran, Rashmi;
- Wener, Mark H;
- Stewart, Lance;
- Veesler, David;
- Diamond, Michael S;
- Greninger, Alexander L;
- Koelle, David M;
- Baker, David
- et al.
Published Web Location
https://doi.org/10.1038/s41587-022-01280-8Abstract
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.
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