The author witnessed the Chilean coup d'etat in 1973. Years later he finds José Emilio Pacheco's, the Mexican bard, report on the event.
Based on an inform written by labor historian Trifonio Delgado Gonzales, this contribution studies the 1940s-1950s Oruro (Bolivia) urban space development and growth. Editor Guillermo Delgado-P. offers an urban anthropological introduction to the document contextualing it within Latin American urban and population growth at the time.
There are 50 billion pieces of litter in the U.S. alone. Grass fields contribute to this problem because picnickers tend to leave trash on the field. We propose building a robot that can autonomously navigate, identify, and pick up trash in parks. To autonomously navigate the park, we used a Spanning Tree Coverage (STC) algorithm to generate a coverage path the robot could follow. To navigate this path, we successfully used Real-Time Kinematic (RTK) GPS, which provides a centimeter-level reading every second. For computer vision, we utilized the ResNet50 Convolutional Neural Network (CNN), which detects trash with 94.52% accuracy. For trash pickup, we tested multiple design concepts. We select a new pickup mechanism that specifically targets the trash we encounter on the field. Our solution achieved an overall success rate of 80%, demonstrating that autonomous trash pickup robots on grass fields are a viable solution.
Translation fidelity relies on accurate aminoacylation of transfer RNAs (tRNAs) by aminoacyl-tRNA synthetases (AARSs). AARSs specific for alanine (Ala), leucine (Leu), serine, and pyrrolysine do not recognize the anticodon bases. Single nucleotide anticodon variants in their cognate tRNAs can lead to mistranslation. Human genomes include both rare and more common mistranslating tRNA variants. We investigated three rare human tRNALeu variants that mis-incorporate Leu at phenylalanine or tryptophan codons. Expression of each tRNALeu anticodon variant in neuroblastoma cells caused defects in fluorescent protein production without significantly increased cytotoxicity under normal conditions or in the context of proteasome inhibition. Using tRNA sequencing and mass spectrometry we confirmed that each tRNALeu variant was expressed and generated mistranslation with Leu. To probe the flexibility of the entire genetic code towards Leu mis-incorporation, we created 64 yeast strains to express all possible tRNALeu anticodon variants in a doxycycline-inducible system. While some variants showed mild or no growth defects, many anticodon variants, enriched with G/C at positions 35 and 36, including those replacing Leu for proline, arginine, alanine, or glycine, caused dramatic reductions in growth. Differential phenotypic defects were observed for tRNALeu mutants with synonymous anticodons and for different tRNALeu isoacceptors with the same anticodon. A comparison to tRNAAla anticodon variants demonstrates that Ala mis-incorporation is more tolerable than Leu at nearly every codon. The data show that the nature of the amino acid substitution, the tRNA gene, and the anticodon are each important factors that influence the ability of cells to tolerate mistranslating tRNAs.
Long noncoding RNAs (LncRNAs) are key regulators of gene expression and can mediate their effects in both the nucleus and cytoplasm. Some of the best-characterized lncRNAs are localized within the nucleus, where they modulate the nuclear architecture and influence gene expression. In this review, we discuss the role of lncRNAs in nuclear architecture in the context of their gene regulatory functions in innate immunity. Here, we discuss various approaches to functionally characterize nuclear-localized lncRNAs and the challenges faced in the field.
