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Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N -glycans
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https://doi.org/10.1039/c6ob00586aAbstract
Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement towards the α1,3-mannose branch, but a great promiscuity towards the α1,6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucosylated asymmetric N-glycans.
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