Lawrence Berkeley National Laboratory

The whiterot fungus Pleurotus ostreatus actively degrades lignin?the second most abundant biopolymer on Earth and an important biomass component of wood and agricultural waste. This edible oyster mushroom also degrades a variety of polycyclic aromatic hydrocarbons and has potential for in situ bioremediation of contaminated soil. JGI sequenced, assembled, and annotated the haploid Pleurotus ostreatus PC15 strain with 8.7x sequencing depth coverage of the resulting 34.3 megabase draft assembly. Annotation using the JGI Annotation pipeline yielded over 11,000 gene models. Approximately 97percent of over 27,000 sequenced ESTs were mapped to the assembly and supported about one quarter of the predicted gene models. The P. ostreatus assembly and gene families were compared with other basidiomycetes including Phanerochaete chrysosporium, Postia placenta, and Laccaria bicolor.