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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.
- Quardokus, Ellen;
- Saunders, Diane;
- McDonough, Elizabeth;
- Hickey, John;
- Werlein, Christopher;
- Surrette, Christine;
- Rajbhandari, Presha;
- Casals, Anna;
- Tian, Hua;
- Lowery, Lisa;
- Neumann, Elizabeth;
- Björklund, Frida;
- Neelakantan, Taruna;
- Croteau, Josh;
- Wiblin, Anne;
- Fisher, Jeremy;
- Livengood, April;
- Dowell, Karen;
- Silverstein, Jonathan;
- Spraggins, Jeffrey;
- Pryhuber, Gloria;
- Deutsch, Gail;
- Ginty, Fiona;
- Nolan, Garry;
- Melov, Simon;
- Jonigk, Danny;
- Caldwell, Michael;
- Vlachos, Ioannis;
- Muller, Werner;
- Gehlenborg, Nils;
- Stockwell, Brent;
- Lundberg, Emma;
- Germain, Ronald;
- Camarillo, Jeannie;
- Kelleher, Neil;
- Börner, Katy;
- Radtke, Andrea;
- Snyder, Michael
- et al.
Published Web Location
https://doi.org/10.1038/s41592-023-01846-7Abstract
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
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