Background: Localized mosquito larval habitat management and the use of larvicides have been proposed as important control tools in integrated malaria vector management programs. In order to optimize the utility of these tools, detailed knowledge of the spatial distribution patterns of mosquito larval habitats is crucial. However, the spatial and temporal changes of habitat distribution patterns under different climatic conditions are rarely quantified and their implications to larval control are unknown.Results: Using larval habitat data collected in western Kenya highlands during both dry and rainy seasons of 2003-2005, this study analyzed the seasonal and inter-annual changes in the spatial patterns in mosquito larval habitat distributions. We found that the spatial patterns of larval habitats had significant temporal variability both seasonally and inter-annually.Conclusions: The pattern of larval habitats is extremely important to the epidemiology of malaria because it results in spatial heterogeneity in the adult mosquito population and, subsequently, the spatial distribution of clinical malaria cases. Results from this study suggest that larval habitat management activities need to consider the dynamic nature of malaria vector habitats. © 2009 Li et al; licensee BioMed Central Ltd.