The Asian monsoon region is highly dependent on boreal summer rainfall, which directly impacts the socio-economic stability and welfare of billions of people each year. Precipitation variability over East China has been extensively studied and is known to be characterized by meridional tripole and dipole precipitation structures. In contrast, few studies have focused on precipitation variability over Mainland Southeast Asia (MSEA) and the possible relationship with the variability over East China. Here we focus on how interannual precipitation variability across MSEA during 1983–2017 may be associated with the tripole or dipole patterns using an empirical orthogonal function (EOF) analysis. The first EOF shows a meridional tripole pattern in East China summer precipitation and an in-phase relationship between MSEA and South China precipitation. In contrast, the second EOF shows a meridional dipole pattern in East China precipitation and an out-of-phase relationship between MSEA and South China precipitation. We show that the first EOF mode is a delayed precipitation response to the El Niño-Southern Oscillation (ENSO), while the second EOF mode is a simultaneous precipitation response to the remote influence of the North Atlantic Oscillation (NAO). Therefore, the in-phase or out-of-phase variations in precipitation between MSEA and South China may be used to gauge the relative importance of local Pacific and remote Atlantic influences on Asian monsoon climate.

Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone is a tedious, time-consuming, and costly process. Thus, there is a critical need for single cell technologies to conduct fast and multiplexed functional analyses followed by recovery of the clone of interest. Here, we use droplet microfluidics for functional screening and real-time monitoring of single TCR T cell activation upon recognition of target tumor cells. Notably, our platform includes a tracking system for each clone as well as a sorting procedure with 100% specificity validated by downstream single cell reverse-transcription PCR and sequencing of TCR chains. Our TCR screening prototype will facilitate immunotherapeutic screening and development of T cell therapies.

T cell receptor (TCR) ligand discovery is essential for understanding and manipulating immune responses to tumors. We developed a cell-based selection platform for TCR ligand discovery that exploits a membrane transfer phenomenon called trogocytosis. We discovered that T cell membrane proteins are transferred specifically to target cells that present cognate peptide-major histocompatibility complex (MHC) molecules. Co-incubation of T cells expressing an orphan TCR with target cells collectively presenting a library of peptide-MHCs led to specific labeling of cognate target cells, enabling isolation of these target cells and sequencing of the cognate TCR ligand. We validated this method for two clinically employed TCRs and further used the platform to identify the cognate neoepitope for a subject-derived neoantigen-specific TCR. Thus, target cell trogocytosis is a robust tool for TCR ligand discovery that will be useful for studying basic tumor immunology and identifying new targets for immunotherapy.