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Article
Peer Reviewed

Effect of freezing on the relative escape force of sperm as measured by a laser optical trap†Presented at the 8th World Congress of In Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Japan, September 11 to 15, 1993.†Supported by grant RR-01192 from the National Institutes of Health, Bethesda, Maryland; by grant 000-14-91-C-0134 from the Office of Naval Research, Arlington, Virginia; and by grant DE-FG03-91 ER 61227 from the Department of Energy, Washington, D.C.

UC Irvine Previously Published Works (1995)

Objective

To determine the possible effect of freezing on sperm escape force as measured by laser trap.

Design

Controlled clinical study.

Setting

Normal volunteers, academic setting.

Patients

Normal, healthy volunteers.

Intervention

Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force before and after freezing.

Main outcome measure

Comparison of escape force as a measurement of freezing effects.

Results

Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh (76.1 +/- 31.1 mW) and in frozen-thawed samples (75.6 +/- 40.0 mW) were similar.

Conclusion

Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.

Cover page: Effect of freezing on the relative escape force of sperm as measured by a laser optical trap*†*Presented at the 8th World Congress of In Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Japan, September 11 to 15, 1993.†Supported by grant RR-01192 from the National Institutes of Health, Bethesda, Maryland; by grant 000-14-91-C-0134 from the Office of Naval Research, Arlington, Virginia; and by grant DE-FG03-91 ER 61227 from the Department of Energy, Washington, D.C.

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