The original version of this Article contained errors in Fig. 2b and Table 2. In Fig. 2b, the white circle labels were incorrectly positioned as they referred to scenarios that were used in an earlier version of the Article. In Table 2, the following three sentences were removed from the legend 'The last two calculations are discussed in the "Methods". The first assumes that all dissolved plus the ≈0.3 nmol kg^-1 of particulate iron (measured in the eastern equatorial Pacific³⁰) is bioavailable. The last calculation assumes EUC dissolved iron concentrations from 140° W'. These errors have now been corrected in both the PDF and HTML versions of the Article.

Nitrate persists in eastern equatorial Pacific surface waters because phytoplankton growth fueled by nitrate (new production) is limited by iron. Nitrate isotope measurements provide a new constraint on the controls of surface nitrate concentration in this region and allow us to quantify the degree and temporal variability of nitrate consumption. Here we show that nitrate consumption in these waters cannot be fueled solely by the external supply of iron to these waters, which occurs by upwelling and dust deposition. Rather, a substantial fraction of nitrate consumption must be supported by the recycling of iron within surface waters. Given plausible iron recycling rates, seasonal variability in nitrate concentration on and off the equator can be explained by upwelling rate, with slower upwelling allowing for more cycles of iron regeneration and uptake. The efficiency of iron recycling in the equatorial Pacific implies the evolution of ecosystem-level mechanisms for retaining iron in surface ocean settings where it limits productivity.

Trophic position is a fundamental characteristic of animals, yet it is unknown in many extinct species. In this study, we ground-truth the ¹⁵N/¹⁴N ratio of enameloid-bound organic matter (δ¹⁵N_EB) as a trophic level proxy by comparison to dentin collagen δ¹⁵N and apply this method to the fossil record to reconstruct the trophic level of the megatooth sharks (genus Otodus). These sharks evolved in the Cenozoic, culminating in Otodus megalodon, a shark with a maximum body size of more than 15 m, which went extinct 3.5 million years ago. Very high δ¹⁵N_EB values (22.9 ± 4.4‰) of O. megalodon from the Miocene and Pliocene show that it occupied a higher trophic level than is known for any marine species, extinct or extant. δ¹⁵N_EB also indicates a dietary shift in sharks of the megatooth lineage as they evolved toward the gigantic O. megalodon, with the highest trophic level apparently reached earlier than peak size.