Abstract With the introduction of next-generation sequencing (NGS) technologies, we are facing an exponential increase in the amount of genomic sequence data. The success of all medical and genetic applications of next-generation sequencing critically depends on the existence of computational techniques that can process and analyze the enormous amount of sequence data quickly and accurately. Unfortunately, the current read mapping algorithms have difficulties in coping with the massive amounts of data generated by NGS. We propose a new algorithm, FastHASH, which drastically improves the performance of the seed-and-extend type hash table based read mapping algorithms, while maintaining the high sensitivity and comprehensiveness of such methods. FastHASH is a generic algorithm compatible with all seed-and-extend class read mapping algorithms. It introduces two main techniques, namely Adjacency Filtering, and Cheap K-mer Selection. We implemented FastHASH and merged it into the codebase of the popular read mapping program, mrFAST. Depending on the edit distance cutoffs, we observed up to 19-fold speedup while still maintaining 100% sensitivity and high comprehensiveness.

Many recent advances in genomics and the expectations of personalized medicine are made possible thanks to power of high throughput sequencing (HTS) in sequencing large collections of human genomes. There are tens of different sequencing technologies currently available, and each HTS platform have different strengths and biases. This diversity both makes it possible to use different technologies to correct for shortcomings; but also requires to develop different algorithms for each platform due to the differences in data types and error models. The first problem to tackle in analyzing HTS data for resequencing applications is the read mapping stage, where many tools have been developed for the most popular HTS methods, but publicly available and open source aligners are still lacking for the Complete Genomics (CG) platform. Unfortunately, Burrows-Wheeler based methods are not practical for CG data due to the gapped nature of the reads generated by this method. Here we provide a sensitive read mapper (sirFAST) for the CG technology based on the seed-and-extend paradigm that can quickly map CG reads to a reference genome. We evaluate the performance and accuracy of sirFAST using both simulated and publicly available real data sets, showing high precision and recall rates.

Runtime resource management for many-core systems is increasingly complex. The complexity can be due to diverse workload characteristics with conflicting demands, or limited shared resources such as memory bandwidth and power. Resource management strategies for many-core systems must distribute shared resource(s) appropriately across workloads, while coordinating the high-level system goals at runtime in a scalable and robust manner. To address the complexity of dynamic resource management in many-core systems, state-of-the-art techniques that use heuristics have been proposed. These methods lack the formalism in providing robustness against unexpected runtime behavior. One of the common solutions for this problem is to deploy classical control approaches with bounds and formal guarantees. Traditional control theoretic methods lack the ability to adapt to (1) changing goals at runtime (i.e., self-adaptivity), and (2) changing dynamics of the modeled system (i.e., self-optimization). In this chapter, we explore adaptive resource management techniques that provide self-optimization and self-adaptivity by employing principles of computational self-awareness, specifically reflection. By supporting these self-awareness properties, the system can reason about the actions it takes by considering the significance of competing objectives, user requirements, and operating conditions while executing unpredictable workloads.