On this game, you should move the player to the goal of each stages. The basic storyline as shown below. "Soul mates, Aru and Bee, got a mysterious accident. After they woke up, they realized that they are trapped in one body! Only one of them can control the body at the same time, and they found that they should take turns to control the body. There are some places where only Red can go through, and other places where only Blue can go through. Travel this weird world and find an exit from the world!"   As you can expect through the story, you should control and explore the player to specific goal. The controls are done with "left", "right", "up", and "z" keys. The first three control keys are done with "platform" behavior on Construct 3. "Left" key makes the player move left, "right" key makes the player move right,  and "up" key makes the player jump. On contract, "z" key is a feature for player that changes player's color either red-to-blue or blue-to-red. This part is related to the story that Aru and Bee got trapped in one body changing their body control to themselves.   On each stages there are many blocks and items and each of them has special characteristics. The red block and red trampoline are only available to use when the player is blue. Also, the  blue block and blue trampoline are only available to use when the player is red. Black block and black trampoline are always available for player. If the player meet with any green blocks, the toxic area, player dies and start at the start point. If the player pass the turquoise ring, the portal, it automatically change the player's color.   The interesting part is that the block on each stages keep changes their color for each periods. So that the player should focus really hard so that they can maintain their state. This is the feature that makes the games very challenging and hard. Player would consider those features try to reach the goal for each stages. There are 7 stages and at the end of the stage, as an ending story, Aru and Bee are separated to each others.   The most characteristic part of this games compared with others is definitely very hard. To reach the final goal, the player would die and restart the stage numerous of times. This would make players mad or stressful and somebody can ask this question,  "Why did you made this game?". My answer is "Because it's fun". This can be sound weird but there are many games that makes people much stressful. Getting Over It with Bennett Foddy, cat mario, world hardest game are well-known games for this. People who enjoys games had heard about those games before. Those games makes people's attempt meaningless a lot by making them into initial state, but still people enjoys those games every time. Because those games are really hard to achieve the final goal, it makes the end of the game much worthy. Person who would play this game will also think achieving the final goal is very worthy, and keep try and enjoy the game.   My game contains a concept with portal. Portal is well used concept for various ways in games or even other media. Especially, the game PORTAL is very famous for the concept of portal. People can make any characteristics using portal on their imaginary world. For my game, I used as a concept that the turquoise ring, the portal, would change the character's color itself.   For the first summit, game players gave us many feedback. Based on this feedback things below has added for the final game.   First, we add a tutorial so that people can easily notice for each feature of the blocks and controls. Because we didn't have any explanation before, people had to die a lot to learn for each features. We don't want player to spend their time meaningless so we add the tutorials.   Second, we add visual and auditory elements to show when the world change occur. On the first version, the blocks change on certain same period with notification. Due to this, people suffer for super hardness so we imported audio and made background change so that people could notice the time much efficiently.   Third, we add more stages and set some save points divides as stage. We want people to enjoy the game longer. This make the world bigger but if there's no any check points people's attempt will be too meaningless. Therefore, additional checkpoints would make people less suffering.   Last, we developed our end story much better so that people who reaching the final goal could be more worthy. Some players had dissatisfaction when they end the first version of game. We made more stories and effect receiving peoples demand.

I chose the theme "soulmate", and made a game describing going trip to Rome with soulmate. I wanted to make the player to have choice as they're really on the trip. But on the first game, it was quite boring because there wasn't any surprising events. So I added some random events to make player more fun.

The LiNi0.5Mn1.5O4 spinel is an appealing cathode material for next generation rechargeable Li-ion batteries due to its high operating voltage of ∼4.7 V (vs Li/Li+). Although it is widely believed that the full range of electrochemical cycling involves the redox of Ni(II)/(IV), it has not been experimentally clarified whether Ni(III) exists as the intermediate state or a double-electron transfer takes place. Here, combined with theoretical calculations, we show unambiguous spectroscopic evidence of the Ni(III) state when the LiNi0.5Mn1.5O4 electrode is half charged. This provides a direct verification of single-electron-transfer reactions in LiNi0.5Mn1.5O4 upon cycling, namely, from Ni(II) to Ni(III), then to Ni(IV). Additionally, by virtue of its surface sensitivity, soft X-ray absorption spectroscopy also reveals the electrochemically inactive Ni2+ and Mn2+ phases on the electrode surface. Our work provides the long-awaited clarification of the single-electron transfer mechanism in LiNi0.5Mn1.5O4 electrodes. Furthermore, the experimental results serve as a benchmark for further spectroscopic characterizations of Ni-based battery electrodes.

Sulfuretin is a natural flavonoid found in the plant Rhus verniciflua STOKES. The plant has been traditionally used as medicinal agent for antiviral, cathartic, diaphoretic, anti-rheumatic and sedative activities in East Asia. In this study we isolated and identified sulfuretin from R. verniciflua and investigated its anti-adipogenic activity against 3T3-L1 preadipocytes cells. We evaluated the effects of sulfuretin on the adipogenic transcription factors like peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), fatty acid synthase (FAS), Fabp4, adiponectin and zinc fingerprint protein (Zfp) 521 by gene expression (real-time QPCR) and Western blot analysis. Sulfuretin treatment at Day 0 and 2 showed significant reduction of lipid production in 3T3-L1 cells in concentration dependent manner. Gene expression analysis (real-time PCR) revealed that sulfuretin inhibited the both major adipogenic factors (C/EBPα, C/EBPβ and PPARγ) and minor adipogenic factors (sterol regulatory element-binding protein (SREBP1c), adiponectin, FAS, Fabp4, Zfp423, and Ebf1). Western blot analysis showed the increased expression of β-catenin and suppression of PPARγ after sulfuretin treatment. Overall, sulfuretin is a natural flavonoid having potent anti-adipogenic activity through the suppression of major adipogenic factors C/EBPα, C/EBPβ and PPARγ, which initiate adipogenesis.

In the last 10 years, organic–inorganic hybrid perovskite solar cells have achieved unprecedented advances, to the point where they now exhibit extremely high efficiency. However, long-term stability and areal scalability limitations impede the commercial application of perovskite materials, and appropriate diagnosistic tools have become necessary to evaluate perovskite materials. Characterization of perovskite materials is regularly misinterpretated, due to unique intrinsic and extrinsic factors: degradation from the measurement source, ion migration, phase transition, and separation. Herein, studies on perovskites are reviewed that have used advanced characterization tools to overcome characterization challenges. Cryogenic temperature assisted measurements mitigate degradation or phase transitions induced by the measurement source. In situ measurements can track the variation of perovskite materials depending on external stimuli. Spatial material properties are able to be evaluated by the use of multidimensional mapping techniques. An overview of these advanced characterization tools that can overcome the challenges associated with established tools provides the opportunity for further understanding perovskite materials and solving the remaining challenges on the road to commercialization.

Dissociative adsorption onto a surface introduces dynamic correlations between neighboring sites not found in non-dissociative absorption. We study surface coverage dynamics where reversible dissociative adsorption of dimers occurs on a finite linear lattice. We derive analytic expressions for the equilibrium surface coverage as a function of the number of reactive sites, N, and the ratio of the adsorption and desorption rates. Using these results, we characterize the finite size effect on the equilibrium surface coverage. For comparable N's, the finite size effect is significantly larger when N is even than when N is odd. Moreover, as N increases, the size effect decays more slowly in the even case than in the odd case. The finite-size effect becomes significant when adsorption and desorption rates are considerably different. These finite-size effects are related to the number of accessible configurations in a finite system where the odd-even dependence arises from the limited number of accessible configurations in the even case. We confirm our analytical results with kinetic Monte Carlo simulations. We also analyze the surface-diffusion case where adsorbed atoms can hop into neighboring sites. As expected, the odd-even dependence disappears because more configurations are accessible in the even case due to surface diffusion.

Coenzyme Q10 (CoQ₁₀) protects retinal ganglion cells (RGCs) in experimental retinal ischemia and glaucoma by scavenging reactive oxygen species. We tested whether a diet supplemented with ubiquinol, the reduced form of CoQ₁₀, promotes RGC survival and blocks the apoptotic pathway in ischemic mouse retina induced by acute high intraocular pressure (IOP) elevation. Ubiquinol (1%) treatment significantly promoted RGC survival at 2 weeks after ischemia/reperfusion. The ubiquinol treatment significantly blocked activation of astroglial and microglial cells in the ischemic retina at 2 weeks. While the ubiquinol treatment significantly decreased active Bax protein expression in the ischemic retina, phosphorylation of Bad at serine 112 and Bcl-xL protein expression were preserved in the ubiquinol-treated ischemic retina at 12 h. Consistently, the ubiquinol treatment prevented apoptotic cell death by blocking caspase-3 cleavage. These results suggest that the ubiquinol enhances RGC survival by modulating the Bax/Bad/Bcl-xL-mediated apoptotic pathway in the ischemic retina. Ubiquinol has therapeutic potential for ameliorating elevated IOP-induced ischemic retinal degeneration.

Plant-derived volatile compounds such as terpenes exhibit substantial structural variation and serve multiple ecological functions. Despite their structural diversity, volatile terpenes are generally produced from a small number of core 5- to 20-carbon intermediates. Here, we present unexpected plasticity in volatile terpene biosynthesis by showing that irregular homo/norterpenes can arise from different biosynthetic routes in a tissue specific manner. While Arabidopsis thaliana and other angiosperms are known to produce the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) or its C16-analog (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene by the breakdown of sesquiterpene and diterpene tertiary alcohols in aboveground tissues, we demonstrate that Arabidopsis roots biosynthesize DMNT by the degradation of the C30 triterpene diol, arabidiol. The reaction is catalyzed by the Brassicaceae-specific cytochrome P450 monooxygenase CYP705A1 and is transiently induced in a jasmonate-dependent manner by infection with the root-rot pathogen Pythium irregulare. CYP705A1 clusters with the arabidiol synthase gene ABDS, and both genes are coexpressed constitutively in the root stele and meristematic tissue. We further provide in vitro and in vivo evidence for the role of the DMNT biosynthetic pathway in resistance against P. irregulare. Our results show biosynthetic plasticity in DMNT biosynthesis in land plants via the assembly of triterpene gene clusters and present biochemical and genetic evidence for volatile compound formation via triterpene degradation in plants.

Coenzyme Q10 (CoQ₁₀) protects retinal ganglion cells (RGCs) in experimental retinal ischemia and glaucoma by scavenging reactive oxygen species. We tested whether a diet supplemented with ubiquinol, the reduced form of CoQ₁₀, promotes RGC survival and blocks the apoptotic pathway in ischemic mouse retina induced by acute high intraocular pressure (IOP) elevation. Ubiquinol (1%) treatment significantly promoted RGC survival at 2 weeks after ischemia/reperfusion. The ubiquinol treatment significantly blocked activation of astroglial and microglial cells in the ischemic retina at 2 weeks. While the ubiquinol treatment significantly decreased active Bax protein expression in the ischemic retina, phosphorylation of Bad at serine 112 and Bcl-xL protein expression were preserved in the ubiquinol-treated ischemic retina at 12 h. Consistently, the ubiquinol treatment prevented apoptotic cell death by blocking caspase-3 cleavage. These results suggest that the ubiquinol enhances RGC survival by modulating the Bax/Bad/Bcl-xL-mediated apoptotic pathway in the ischemic retina. Ubiquinol has therapeutic potential for ameliorating elevated IOP-induced ischemic retinal degeneration.