• Peak standing crop (PSC) and residual dry matter (RDM) are the primary measures of production and grazing intensity on California's annual rangelands. • One of the most common methods of monitoring forage metrics is to clip 1-ft2 quadrats. The USDA Forest Service, Bureau of Land Management, universities, and other land managers have been using this methodology since the 1930s. • We used best linear unbiased predictors (BLUEs) to determine 95% confidence intervals for PSC and RDM. For both PSC and RDM, as the number of samples taken increased from 1 to 10, the predictive ability also significantly increased. We found no evidence of increased predictive power past 10 samples.

Allohexaploid (2n = 6x = 42) intermediate wheatgrass (Thinopyrum intermedium), abbreviated IWG, is an outcrossing perennial grass belonging to the tertiary gene pool of wheat. Perenniality would be valuable option for grain production, but attempts to introgress this complex trait from wheat-Thinopyrum hybrids have not been commercially successful. Efforts to breed IWG itself as a dual-purpose forage and grain crop have demonstrated useful progress and applications, but grain yields are significantly less than wheat. Therefore, genetic and physical maps have been developed to accelerate domestication of IWG. Herein, these maps were used to identify quantitative trait loci (QTLs) and candidate genes associated with IWG grain production traits in a family of 266 full-sib progenies derived from two heterozygous parents, M26 and M35. Transgressive segregation was observed for 17 traits related to seed size, shattering, threshing, inflorescence capacity, fertility, stem size, and flowering time. A total of 111 QTLs were detected in 36 different regions using 3826 genotype-by-sequence markers in 21 linkage groups. The most prominent QTL had a LOD score of 15 with synergistic effects of 29% and 22% over the family means for seed retention and percentage of naked seeds, respectively. Many QTLs aligned with one or more IWG gene models corresponding to 42 possible domestication orthogenes including the wheat Q and RHT genes. A cluster of seed-size and fertility QTLs showed possible alignment to a putative Z self-incompatibility gene, which could have detrimental grain-yield effects when genetic variability is low. These findings elucidate pathways and possible hurdles in the domestication of IWG.

Livestock obtain forage by grazing on rangeland. In California annual rangelands, residual dry matter is commonly used to determine proper grazing levels. Rangeland forage biomass and quality can degrade dramatically during the dormant summer period. We examined 25 sites across an annual rainfall gradient (183–492 mm) over 3 contrasting rainfall yr (2015–2017) that varied from 57% to 152% of average annual precipitation. Overall fractional biomass loss was 54.4% (range = 46.5–61.5%) with greater fractional losses occurring in dry years. Biomass losses were related to the amount of peak standing crop and plant composition—both a function of annual precipitation. Fractional seasonal losses from the peak standing biomass in 2015 = 962 kg/ha (61.5% seasonal; 9.7% monthly), 2016 = 1 541 kg/ha (55.0% seasonal; 8.7%monthly) and 2017 = 1 923 kg/ha (46.5% seasonal; 7.3%, monthly). Forage quality metrics were strongly affected by summer weathering processes. Crude protein concentrations decreased by 33.6%, 27.7%, and 21.0% in 2015, 2016, and 2017, respectively. In contrast, relative concentrations of fiber and lignin (acid detergent fiber [ADF] = cellulose + lignin) and in the weathered biomass showed increases for ADF: 44.6% (2015), 32.2% (2016), and 24.1% (2017). Increased lignin varied: 3.4% in 2015, 23.9% in 2016, and 28.0% in 2017. While ADF and lignin concentrations (weathered biomass, kg/ha) increased during the weathering process, the standing stock decreased by 39.3% (ADF) and 46.6% (lignin), compared with overall weathered biomass loss of 54.4% and CP loss of 67.1%. The significant loss of aboveground biomass and forage quality as weathering processes occurred throughout the dry summer period affects livestock grazing strategies. Forage biomass and nutrient losses through the dry season should be considered when determining grazing strategies to achieve proper residual dry matter levels and nutrient supplementation regimes before the onset of the rainy season.

Importance

Posttraumatic stress disorder (PTSD) is a prevalent, serious public health concern, particularly in the military. The identification of genetic risk factors for PTSD may provide important insights into the biological foundation of vulnerability and comorbidity.

Objective

To discover genetic loci associated with the lifetime risk for PTSD in 2 cohorts from the Army Study to Assess Risk and Resilience in Servicemembers (Army STARRS).

Design, setting, and participants

Two coordinated genome-wide association studies of mental health in the US military contributed participants. The New Soldier Study (NSS) included 3167 unique participants with PTSD and 4607 trauma-exposed control individuals; the Pre/Post Deployment Study (PPDS) included 947 unique participants with PTSD and 4969 trauma-exposed controls. The NSS data were collected from February 1, 2011, to November 30, 2012; the PDDS data, from January 9 to April 30, 2012. The primary analysis compared lifetime DSM-IV PTSD cases with trauma-exposed controls without lifetime PTSD. Data were analyzed from March 18 to December 27, 2015.

Main outcomes and measures

Association analyses for PTSD used logistic regression models within each of 3 ancestral groups (European, African, and Latino American) by study, followed by meta-analysis. Heritability and genetic correlation and pleiotropy with other psychiatric and immune-related disorders were estimated.

Results

The NSS population was 80.7% male (6277 of 7774 participants; mean [SD] age, 20.9 [3.3] years); the PPDS population, 94.4% male (5583 of 5916 participants; mean [SD] age, 26.5 [6.0] years). A genome-wide significant locus was found in ANKRD55 on chromosome 5 (rs159572; odds ratio [OR], 1.62; 95% CI, 1.37-1.92; P = 2.34 × 10-8) and persisted after adjustment for cumulative trauma exposure (adjusted OR, 1.64; 95% CI, 1.39-1.95; P = 1.18 × 10-8) in the African American samples from the NSS. A genome-wide significant locus was also found in or near ZNF626 on chromosome 19 (rs11085374; OR, 0.77; 95% CI, 0.70-0.85; P = 4.59 × 10-8) in the European American samples from the NSS. Similar results were not found for either single-nucleotide polymorphism in the corresponding ancestry group from the PPDS sample, in other ancestral groups, or in transancestral meta-analyses. Single-nucleotide polymorphism-based heritability was nonsignificant, and no significant genetic correlations were observed between PTSD and 6 mental disorders or 9 immune-related disorders. Significant evidence of pleiotropy was observed between PTSD and rheumatoid arthritis and, to a lesser extent, psoriasis.

Conclusions and relevance

In the largest genome-wide association study of PTSD to date, involving a US military sample, limited evidence of association for specific loci was found. Further efforts are needed to replicate the genome-wide significant association with ANKRD55-associated in prior research with several autoimmune and inflammatory disorders-and to clarify the nature of the genetic overlap observed between PTSD and rheumatoid arthritis and psoriasis.