Since the initiation of the U.S.-China Clean Energy Research Center-Building Energy Efficiency (CERC-BEE) cool roof research collaboration between the Lawrence Berkeley National Laboratory Heat Island Group and Chinese institutions in 2010, new cool surface credits (insulation trade- offs) have been adopted in Chinese building energy efficiency standards, industry standards, and green building standards. JGJ 75-2012: Design Standard for Energy Efficiency of Residential Buildings in Hot Summer and Warm Winter Zone became the first national level standard to provide cool surface credits. GB/T 50378-2014: Assessment Standard for Green Building is the first national level green building standard that offers points for heat island mitigation. JGJ/T 359-2015: Technical Specification for Application of Architectural Reflective Thermal Insulation Coating is the first industry standard that offers cool coating credits for both public and residential buildings in all hot-summer climates (Hot Summer/Cold Winter, Hot Summer/Warm Winter). As of December 2015, eight provinces or municipalities in hot-summer regions have credited cool surfaces credits in their residential and/or public building design standards; five other provinces or municipalities in hot-summer regions recommend, but do not credit, the use of cool surfaces in their building design standards. Cool surfaces could be further advanced in China by including cool roof credits for residential and public building energy efficiency standards in all hot-summer regions; developing a standardized process for natural exposure and aged-property rating of cool roofing products; and adapting the U.S.-developed laboratory aging process for roofing materials to replicate solar reflectance changes induced by natural exposure in China.

This study presents the experimental measurement of the energy consumption of three top-floor air-conditioned rooms in a typical office building in Chongqing, which is a mountainous city in the hot-summer and cold-winter zone of China, to examine the energy performance of white and sedum-tray garden roofs. The energy consumption of the three rooms was measured from September 2014 to September 2015 by monitoring the energy performance (temperature distributions of the roofs, evaporation, heat fluxes, and energy consumption) and indoor air temperature. The rooms had the same construction and appliances, except that one roof top was black, one was white, and one had a sedum-tray garden roof. This study references the International Performance Measurement and Verification Protocol (IPMVP) to calculate and compare the energy savings of the three kinds of roofs. The results indicate that the energy savings ratios of the rooms with the sedum-tray garden roof and with the white roof were 25.0% and 20.5%, respectively, as compared with the black-roofed room, in the summer; by contrast, the energy savings ratios were −9.9% and −2.7%, respectively, in the winter. Furthermore, Annual conditioning energy savings of white roof (3.9 kWh/m2) were 1.6 times the energy savings for the sedum-tray garden roof. It is evident that white roof is a preferable choice for office buildings in Chongqing. Additionally, The white roof had a reflectance of 0.58 after natural aging owing to the serious air pollution worsened its thermal performance, and the energy savings reduced by 0.033 kWh/m2·d. Evaporation was also identified to have a significant effect on the energy savings of the sedum-tray garden roof.

Cry toxins have been widely used in genetically modified organisms for pest control, raising public concern regarding their effects on the natural environment and food safety. In this work, a phage-mediated competitive chemiluminescent immunoassay (c-CLIA) was developed for determination of Cry1Ab toxin using anti-idiotypic camel nanobodies. By extracting RNA from camels' peripheral blood lymphocytes, a naive phage-displayed nanobody library was established. Using anti-Cry1Ab toxin monoclonal antibodies (mAbs) against the library for anti-idiotypic antibody screening, four anti-idiotypic nanobodies were selected and confirmed to be specific for anti-Cry1Ab mAb binding. Thereafter, a c-CLIA was developed for detection of Cry1Ab toxin based on anti-idiotypic camel nanobodies and employed for sample testing. The results revealed a half-inhibition concentration of developed assay to be 42.68 ± 2.54 ng/mL, in the linear range of 10.49-307.1 ng/mL. The established method is highly specific for Cry1Ab recognition, with negligible cross-reactivity for other Cry toxins. For spiked cereal samples, the recoveries of Cry1Ab toxin ranged from 77.4% to 127%, with coefficient of variation of less than 9%. This study demonstrated that the competitive format based on phage-displayed anti-idiotypic nanobodies can provide an alternative strategy for Cry toxin detection.