The notion that closely related species resemble each other in ecological niche space (i.e., phylogenetic dependence) has been a long-standing, contentious paradigm in evolutionary biology, the incidence of which is important for predicting the ecosystem-level effects of species loss. Despite being examined across a multitude of terrestrial taxa, many aspects of niche conservatism have yet to be explored in marine species, especially for characteristics related to resource use and trophic behavior (Eltonian niche characteristics, ENCs). We combined ENCs derived from stable isotope ratios at assemblage- and species-levels with phylogenetic comparative methods, to test the hypotheses that benthic marine fishes (1) exhibit similar assemblage-wide ENCs regardless of geographic location and (2) display phylogenetically dependent ENCs at the species level. We used a 12-species sub-set of the monophyletic group Rajidae sampled from three independent assemblages (Central California, Gulf of Alaska, and Northwest Atlantic), which span two ocean basins. Assemblage-level ENCs implied low trophic diversity and high evenness, suggesting that Rajidae assemblages may exhibit a well-defined trophic role, a trend consistent regardless of geographic location. At the species level, we found evidence for phylogenetic dependence of ENCs relating to trophic diversity (i.e., isotopic niche width; SEAc). Whether individuals can be considered functional equivalents across assemblages is hard to ascertain because we did not detect a significant phylogenetic signal for ENCs relating to trophic function (e.g., trophic position). Thus, additional, complimentary approaches are required to further examine the phylogenetic dependence of species functionality. Our approach illustrates the potential of stable isotope-derived niche characteristics to provide insight on macroecological processes occurring across evolutionary time, which could help predict how assemblages may respond to the effects of species loss.

Rationale

Hypokalemia occurs in up to 20% of hospitalized patients and is associated with increased incidence of ventricular and atrial fibrillation. It is unclear whether these differing types of arrhythmia result from direct and perhaps distinct effects of hypokalemia on cardiomyocytes.

Objective

To investigate proarrhythmic mechanisms of hypokalemia in ventricular and atrial myocytes.

Methods and results

Experiments were performed in isolated rat myocytes exposed to simulated hypokalemia conditions (reduction of extracellular [K⁺] from 5.0 to 2.7 mmol/L) and supported by mathematical modeling studies. Ventricular cells subjected to hypokalemia exhibited Ca²⁺ overload and increased generation of both spontaneous Ca²⁺ waves and delayed afterdepolarizations. However, similar Ca²⁺-dependent spontaneous activity during hypokalemia was only observed in a minority of atrial cells that were observed to contain t-tubules. This effect was attributed to close functional pairing of the Na⁺-K⁺ ATPase and Na⁺-Ca²⁺ exchanger proteins within these structures, as reduction in Na⁺ pump activity locally inhibited Ca²⁺ extrusion. Ventricular myocytes and tubulated atrial myocytes additionally exhibited early afterdepolarizations during hypokalemia, associated with Ca²⁺ overload. However, early afterdepolarizations also occurred in untubulated atrial cells, despite Ca²⁺ quiescence. These phase-3 early afterdepolarizations were rather linked to reactivation of nonequilibrium Na⁺ current, as they were rapidly blocked by tetrodotoxin. Na⁺ current-driven early afterdepolarizations in untubulated atrial cells were enabled by membrane hyperpolarization during hypokalemia and short action potential configurations. Brief action potentials were in turn maintained by ultra-rapid K⁺ current (I_Kur); a current which was found to be absent in tubulated atrial myocytes and ventricular myocytes.

Conclusions

Distinct mechanisms underlie hypokalemia-induced arrhythmia in the ventricle and atrium but also vary between atrial myocytes depending on subcellular structure and electrophysiology.

Understanding the factors shaping patterns of ecological resilience is critical for mitigating the loss of global biodiversity. Throughout aquatic environments, highly mobile predators are thought to serve as important vectors of energy between ecosystems thereby promoting stability and resilience. However, the role these predators play in connecting food webs and promoting energy flow remains poorly understood in most contexts. Using carbon and nitrogen isotopes, we quantified the use of several prey resource pools (small oceanic forage, large oceanics, coral reef, and seagrass) by 17 species of elasmobranch fishes (n = 351 individuals) in The Bahamas to determine their functional diversity and roles as ecosystem links. We observed remarkable functional diversity across species and identified four major groups responsible for connecting discrete regions of the seascape. Elasmobranchs were responsible for promoting energetic connectivity between neritic, oceanic and deep-sea ecosystems. Our findings illustrate how mobile predators promote ecosystem connectivity, underscoring their functional significance and role in supporting ecological resilience. More broadly, strong predator conservation efforts in developing island nations, such as The Bahamas, are likely to yield ecological benefits that enhance the resilience of marine ecosystems to combat imminent threats such as habitat degradation and climate change.