Soil compaction is a common consequence of forestry traffic traversing unprotected, moist soils; it decreases porosity and affects hydraulic conductivity even in coarse-textured soils. The aim here was to study root-zone hydrology and vegetation in three microsites (in, between, and beside wheel tracks) 4 to 5 yr after forwarder traffic, on stony and sandy till soils in two clearcuts in northern Sweden. Measurements of soil volumetric water content (VWC), vegetation indicators and one-dimensional hydrological modeling (Hydrus-1D) of wheel tracks and undisturbed soil were conducted. Soil VWC was monitored hourly during 2017 and 2018 in three or four plots along a slope on each site. Soil VWC was also measured once with a portable sensor in 117 plots along two slopes at each site, where the vegetation was recorded and analyzed using Ellenberg indicator indexes. Soil VWC was highest in wheel tracks and lowest between tracks; this was corroborated by the species composition in the wheel tracks (Ellenberg indicator for soil moisture). Bare soil was more frequent in wheel tracks and between tracks than in undisturbed soil. The model simulations indicated that the changed soil hydraulic properties influenced the VWC results in the wheel tracks. However, the differences in average pressure heads in the root zone were small between the microsites and only apparent during dry periods. In the wheel tracks, air-filled porosity was <0.10 m3 m-3, indicating insufficient soil aeration during 82% (Site T) and 23% (Site R) of the 2017 growing season. Insufficient aeration could be one explanation for the presence of some still unvegetated areas.

[This corrects the article DOI: 10.1371/journal.pgen.1006481.].

ATP6V1H is a component of a large protein complex with vacuolar ATPase (V-ATPase) activity. We identified two generations of individuals in which short stature and osteoporosis co-segregated with a mutation in ATP6V1H. Since V-ATPases are highly conserved between human and zebrafish, we generated loss-of-function mutants in atp6v1h in zebrafish through CRISPR/Cas9-mediated gene knockout. Homozygous mutant atp6v1h zebrafish exhibited a severe reduction in the number of mature calcified bone cells and a dramatic increase in the expression of mmp9 and mmp13. Heterozygous adults showed curved vertebra that lack calcified centrum structure and reduced bone mass and density. Treatment of mutant embryos with small molecule inhibitors of MMP9 and MMP13 significantly restored bone mass in the atp6v1h mutants. These studies have uncovered a new, ATP6V1H-mediated pathway that regulates bone formation, and defines a new mechanism of disease that leads to bone loss. We propose that MMP9/MMP13 could be therapeutic targets for patients with this rare genetic disease.

Regenerative medicine holds great promise for both degenerative diseases and traumatic tissue injury which represent significant challenges to the health care system. Hearing loss, which affects hundreds of millions of people worldwide, is caused primarily by a permanent loss of the mechanosensory receptors of the inner ear known as hair cells. This failure to regenerate hair cells after loss is limited to mammals, while all other non-mammalian vertebrates tested were able to completely regenerate these mechanosensory receptors after injury. To understand the mechanism of hair cell regeneration and its association with regeneration of other tissues, we performed a guided mutagenesis screen using zebrafish lateral line hair cells as a screening platform to identify genes that are essential for hair cell regeneration, and further investigated how genes essential for hair cell regeneration were involved in the regeneration of other tissues. We created genetic mutations either by retroviral insertion or CRISPR/Cas9 approaches, and developed a high-throughput screening pipeline for analyzing hair cell development and regeneration. We screened 254 gene mutations and identified 7 genes specifically affecting hair cell regeneration. These hair cell regeneration genes fell into distinct and somewhat surprising functional categories. By examining the regeneration of caudal fin and liver, we found these hair cell regeneration genes often also affected other types of tissue regeneration. Therefore, our results demonstrate guided screening is an effective approach to discover regeneration candidates, and hair cell regeneration is associated with other tissue regeneration.

The focus of this paper is to briefly discuss the major advances in scientific thinking regarding: a) processes governing the fate and transport of mercury in the environment; b) advances in measurement methods; and c) how these advances in knowledge fit in within the context of the Minamata Convention on Mercury. Details regarding the information summarized here can be found in the papers associated with this Virtual Special Issue of STOTEN.