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Rapid Sequencing of Complete env Genes from Primary HIV-1 Samples
- Smith, Melissa Laird;
- Murrell, Ben;
- Eren, Kemal;
- Ignacio, Caroline;
- Landais, Elise;
- Weaver, Steven;
- Phung, Pham;
- Ludka, Colleen;
- Hepler, Lance;
- Caballero, Gemma;
- Pollner, Tristan;
- Guo, Yan;
- Richman, Douglas;
- Network, The IAVI Protocol C Investigators The IAVI African HIV Research;
- Poignard, Pascal;
- Paxinos, Ellen E;
- Pond, Sergei L Kosakovsky;
- Smith, Davey M
- et al.
Published Web Location
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822884/No data is associated with this publication.
Abstract
The ability to study rapidly evolving viral populations has been constrained by the read length of next-generation sequencing approaches and the sampling depth of single-genome amplification methods. Here, we develop and characterize a method using Pacific Biosciences' Single Molecule, Real-Time (SMRT®) sequencing technology to sequence multiple, intact full-length human immunodeficiency virus-1 env genes amplified from viral RNA populations circulating in blood, and provide computational tools for analyzing and visualizing these data.
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