Three experiments investigated the frame of reference used in memory to represent vertical spatial layouts perceiv-able from a single viewpoint. We tested for the selection of three different reference systems: the body orientation, the visualvertical of the surrounding room, and the direction of gravity. Participants learned and retrieved differently colored objects ona vertical board with body and room orientations varying in relation to gravity and each other systematically. Across all threeexperiments participants were quicker or more accurate in memory recall when they saw the vertical spatial layout in the sameorientation in relation to their body vertical as during learning, irrespective of the direction of gravity or visual room upright.These results indicate that spatial long-term memories for small-scale vertical relations are mainly defined in an egocentricreference system with respect to the body vertical despite the availability of alternative highly salient allocentric referencedirections.