The aim of our study was to evaluate the community structure and species composition of seeds present in the transient seed bank in two areas of Atlantic forest fragment in São Paulo: an area where the bamboo is overabundant and another without bamboos, both located in a secondary forest fragment. Our hypothesis is that bamboo overabundance influences the availability and species diversity of seeds in the transient soil seed bank, what, in tum, can influence the potential for natural forest recovery. We collected 45 samples of litter in each site and sorted, quantified and identified seeds to the most precise taxonomic levels. We found lower species richness and higher proportion of exclusive species in the area where bamboo is overabundant, but there was no difference in the abundance of seeds in the transient seed bank between areas. Our results show that seed distribution in the bamboo overabundant area was more limited than in control area because 50% of collected samples did not present any seeds. It was not possible to conclude whether the reduction on species richness of the transient seed bank was a result of the lower density of tree species or just a side effect of the overabundance of bamboo. The decrease in species richness and seed dispersal limitation in transient seed bank found in bamboo overabundant area suggest the decreased contribution of this pathway regeneration of vegetation.