Spatial variations in the N2O emissions and denitrification potential of riparian buffer strips (RBS) in a polluted river were examined. The river received large pollutant inputs from urban runoff and wastewater discharge, resulting in impaired water quality in the river and downstream reservoir. The potential for nitrogen removal by RBS was evaluated by measuring in situ N2O emission fluxes in static closed chambers and sediment denitrification potentials with acetylene inhibition techniques. The results showed that N2O emission fluxes decreased from the upstream (16.39 μg/(m2·h)) to downstream (0.30 μg/(m2·h)) sites and from the water body to upland sites. The trend in decreasing N2O emission fluxes in the downstream direction was mainly associated with sediment/soil textures (clay loam→sandy soil) and sediment/soil water contents and was also related to the vegetation along the RBS and nutrients in the sediments/soils. The correlation coefficient was highest (r=0.769) between the N2O emission flux and sediment/soil water content. Sediment/soil denitrification potentials under N-amended and ambient conditions were higher (highest 32.86 mg/(kg·h)) for the upstream sites, which were consistent with in situ N2O flux rates. © 2013 Copyright Taylor and Francis Group, LLC.