We present a data set reporting the checklist of the species of the phylum Rotifera for Italy, updating the one previously published in the series ‘Checklist delle Specie della Fauna d'Italia’ in 1995. The records of the updated checklist refer to the 483 taxa at the species and subspecies level currently known from national Italian territories (119 Bdelloidea, 362 Monogononta, 2 Seisonacea) at the regional level (22 terrestrial and nine marine geographical units). The records refer to various freshwater, limno-terrestrial, and marine coastal habitats. The previous checklist reported 245 taxa (54 Bdelloidea, 189 Monogononta, 2 Seisonacea): three taxa were removed because currently considered not valid and 241 were added, scanning 21 papers we found that were published between 1993 and 2020, expanding the regional records and including four papers older than 1993 with overlooked records in the previous checklist. The Rotifera data are part of the updated Checklist of the Italian Fauna, which is viewable on the LifeWatch Italy platform at https://www.lifewatchitaly.eu/en/initiatives/checklist-fauna-italia-en/checklist and is freely available on the LifeWatch Italy Data Portal (https://dataportal.lifewatchitaly.eu/data). The checklist will be dynamically updated with new records; this paper describes the state of the art of the data set regarding Rotifera on May 2021.