We examined the spatial and temporal distributions of fishes at a reference and three restored marshes between April 1998 and July 1999 in the Sacramento–San Joaquin Delta, California, to determine the factors that influence fish assemblages in space and time. Shallow-water fishes were sampled using beach seines with and without block-net enclosures in open-water shoals and submerged aquatic vegetation (SAV). Purse seining was used to sample fishes in deep water, including offshore, tidal slough, and marsh-edge habitats. Overall, fish assemblages in reference and restored marshes were dominated by introduced species. One-way analysis of similarity (ANOSIM) did not reveal study site differences in fish assemblages in either data set. However, nonmetric multi-dimensional scaling (NMS) and ANOSIM tests of the shallow-water collections revealed differences in fish assemblages using habitats with and without SAV. Introduced fishes, including predatory centrarchid fishes, were abundant in SAV. NMS and ANOSIM tests of the deep-water collections revealed differences in fish assemblages between offshore and nearshore (marsh-edge and tidal slough) habitats. Notably, native fishes were abundant in tidal sloughs. Temporal analyses revealed a suite of species more common in winter and spring, versus another group of introduced species that were more common in summer and fall. Our study findings indicate that newly restored habitats in the Sacramento–San Joaquin Delta will be invaded by introduced fishes. To promote native fish habitat, restoration planning should focus on areas and regions of the Delta where tidal marshes can be restored with little intervention, and where invasive SAV is less likely to colonize.

Recruitment of estuarine organisms can vary dramatically from year to year with abiotic and biotic conditions. The San Francisco Estuary (California, USA) supports a dynamic ecosystem that receives freshwater flow from numerous tributaries that drain one of the largest watersheds in western North America. In this study, we examined distribution and habitat use of two forage fish larvae of management interest, Longfin Smelt Spirinchus thaleichthys and Pacific Herring Clupea pallasii, during a low-flow and a high-flow year to better understand how their rearing locations (region and habitat) may affect their annual recruitment variability. During the low-flow year, larval and post-larval Longfin Smelt were distributed landward, where suitable salinity overlapped with spawning habitats. During the high-flow year, larval Longfin Smelt were distributed seaward, with many collected in smaller tributaries and shallow habitats of San Francisco Bay. Local spawning and advection from seaward habitats were speculated to be the primary mechanisms that underlie larval Longfin Smelt distribution during the high-flow year. Larval Pacific Herring were more abundant seaward in both years, but a modest number of larvae were also found landward during the low-flow year. Larval Pacific Herring abundance was lower overall in the high-flow year, suggesting advection out of the area or poor recruitment. Future monitoring and conservation efforts for Longfin Smelt and Pacific Herring should recognize that potential mechanisms underlying their recruitment can vary broadly across the San Francisco Estuary in any given year, which suggests that monitoring and research of these two species expand accordingly with hydrologic conditions that are likely to affect their spawning and larval rearing distributions.