Precipitation is a key driver of ecosystem development, and terrestrial ecosystems are experiencing important changes in the amount and seasonality of precipitation, which is expected to go on well into the next century. Precipitation affects soil moisture, and there is still uncertainty about how changing soil moisture regimes will affect carbon and nitrogen cycling, and how these climate change impacts will interact with changing plant species diversity to affect the dynamics of soil organic matter. We studied carbon and nitrogen cycling in two Mediterranean systems, a natural precipitation gradient of California grasslands, and a precipitation manipulation experiment. The precipitation gradient focuses on three sites across California that experience precipitation ranging from low (~300 mm precipitation/year) to a wet precipitation regime (~2160 mm precipitation/year). The precipitation manipulation experiment is set up in a random block design at the wettest site in the gradient. It has three treatments: ambient precipitation, additional precipitation in the winter, and additional precipitation in the spring. We collected samples from across the natural precipitation gradient to 1m and also at the precipitation experiment to 3m after 20 years of manipulation. This sampling design addresses key questions regarding how changes in the amount and seasonality of precipitation affect soil organic matter stability and turnover of soils below and above 1 meter. At the precipitation gradient, we determined changes in total elemental concentrations of soil carbon and nitrogen, stable isotope composition (δ13C, δ15N), and composition of soil organic matter (SOM) as measured through Diffuse Reflectance Infrared Fourier Transformed Spectroscopy (DRIFTS) to 1m soil depth. We measured carbon persistence in soil organic matter (SOM) based on beta (β), a parameter based on the slope of carbon isotope composition across depth and proxy for turnover. Further, we examined the relationship between δ15N and C:N values to infer SOM’s degree of microbial processing. As expected, we measured the greatest carbon stock at the surface of our wettest site, but carbon stocks in subsoils converged at the wet and dry sites. Soils at depth (>30cm) at the wettest site had the lowest C:N ratio and highest δ15N values with the greatest proportion of simple plant-derived organic matter, as determined DRIFTS. These results suggest differing stabilization mechanisms of organic matter at depth across our study sites. We infer that the greatest stability was conferred by associations with reactive minerals at depth in our wettest site. In contrast, organic matter at our driest site was subject to the most microbial processing. Results from this study demonstrate that precipitation patterns have important implications for deep soil carbon storage and composition, suggesting the vulnerability of deep SOM to climate change induced alterations in precipitation patterns. At the precipitation experiment, we found that the winter precipitation addition resulted in the largest cumulative (0-300cm) C stock. However, we found evidence for vertical translocation of carbon to deep soil layers, specifically of plant-derived carbon, with both winter and spring additions of precipitation. Winter addition of precipitation also resulted in the highest subsoil carbon stock compared to the ambient and spring treatments. Overall, added winter precipitation led to the best conditions for carbon accumulation since the added precipitation coincides with lower temperatures and improved growing conditions at our field site. When we zoomed into surface soils at the precipitation experiment, we found greater surface carbon stocks with winter addition of precipitation. We also found weakened associations with short-range order iron and aluminum oxides in the winter and spring treatments. However, we did see significant and positive associations in only the winter treatment between short range order oxides and complex plant matter and microbially associated OM. The conclusions of this study suggest that over decadal time scales, changing precipitation amount and seasonality could affect SOM accumulation and persistence in grassland ecosystems.