The initial stages of a disease outbreak can determine the magnitude of the ensuing epidemic. Though rarely tested in unison, two factors with important consequences for the transmission dynamics of infectious agents are the collective traits of the susceptible population and the individual traits of the index case (i.e. 'patient zero'). Here, we test whether the personality composition of a social group can explain horizontal transmission dynamics of cuticular bacteria using the social spider Stegodyphus dumicola We exposed focal spiders of known behavioural phenotypes with a GFP-transformed cuticular bacterium (Pantoea sp.) and placed them in groups of 10 susceptible individuals (i.e. those with no experience with this bacterium). We measured bacterial transmission to groups composed of either all shy spiders, 10% bold spiders or 40% bold spiders. We found that colonies with 40% bold spiders experienced over twice the incidence of transmission compared to colonies with just 10% bold individuals after only 24 h of interaction. Colonies of all shy spiders experienced an intermediate degree of transmission. Interestingly, we did not detect an effect of the traits of the index case on transmission. These data suggest that the phenotypic composition of the susceptible population can have a greater influence on the degree of early transmission events than the traits of the index case.

Amphibians are ideal for studying visual system evolution because their biphasic (aquatic and terrestrial) life history and ecological diversity expose them to a broad range of visual conditions. Here, we evaluate signatures of selection on visual opsin genes across Neotropical anurans and focus on three diurnal clades that are well-known for the concurrence of conspicuous colors and chemical defense (i.e., aposematism): poison frogs (Dendrobatidae), Harlequin toads (Bufonidae: Atelopus), and pumpkin toadlets (Brachycephalidae: Brachycephalus). We found evidence of positive selection on 44 amino acid sites in LWS, SWS1, SWS2, and RH1 opsin genes, of which one in LWS and two in RH1 have been previously identified as spectral tuning sites in other vertebrates. Given that anurans have mostly nocturnal habits, the patterns of selection revealed new sites that might be important in spectral tuning for frogs, potentially for adaptation to diurnal habits and for color-based intraspecific communication. Furthermore, we provide evidence that SWS2, normally expressed in rod cells in frogs and some salamanders, has likely been lost in the ancestor of Dendrobatidae, suggesting that under low-light levels, dendrobatids have inferior wavelength discrimination compared to other frogs. This loss might follow the origin of diurnal activity in dendrobatids and could have implications for their behavior. Our analyses show that assessments of opsin diversification in across taxa could expand our understanding of the role of sensory system evolution in ecological adaptation.