This guideline is intended to provide information relevant to the specification of triple- pane insulating glass (IG) units where the thickness of lite number 2 (center lite) is less than or equal to the thickness of lite numbers 1 and 3. Procedures and charts are presented to determine the load resistance (LR) of common soda-lime glass thicknesses from 0.7 mm (3/128 in.) to 1.8 mm (9/128 in.) exposed to a uniform lateral load of short or long duration, for a 0.008 probability of breakage. Deflection under load, horizontal self-weight deflection, and natural frequency procedures and charts are also included to aide in the selection of glass thickness to meet manufacturing and/or loading criteria determined by the user.

Background

Recently, measurement of RNA at single cell resolution has yielded surprising insights. Methods for single-cell RNA sequencing (scRNA-seq) have received considerable attention, but the broad reliability of single cell methods and the factors governing their performance are still poorly known.

Results

Here, we conducted a large-scale control experiment to assess the transfer function of three scRNA-seq methods and factors modulating the function. All three methods detected greater than 70% of the expected number of genes and had a 50% probability of detecting genes with abundance greater than 2 to 4 molecules. Despite the small number of molecules, sequencing depth significantly affected gene detection. While biases in detection and quantification were qualitatively similar across methods, the degree of bias differed, consistent with differences in molecular protocol. Measurement reliability increased with expression level for all methods and we conservatively estimate measurements to be quantitative at an expression level greater than ~5-10 molecules.

Conclusions

Based on these extensive control studies, we propose that RNA-seq of single cells has come of age, yielding quantitative biological information.