The effect of asperity flattening of a rough spherical contact during cyclic loading is investigated experimentally. Two types of surfaces are examined; the first is an “as-manufactured” isotropic surface and the second a smooth “laser-polished” surface. Both the surfaces exhibit a large amount of hysteresis of the load–displacement curve during the first load–unload cycles. This hysteresis is found to decrease as a function of the number of load cycles. A comparison of the experimental results with results obtained from a numerical model for a rough spherical contact shows good correlation. The model shows that for rough surfaces the total displacement is a function of the contacting asperities while for smooth surfaces the main contribution comes from the bulk displacement.